Neurofilament Proteins Are Distributed in a Diminishing Proximodistal Gradient Along Rat Sciatic Nerve

Neurofilament (NF) proteins are distributed in a diminishing proximodistal gradient along rat sciatic nerve when compared with total noncollagen or other proteins in nerve. About a twofold decline of NF proteins can be detected by quantitating nerve proteins that have been separated by gel electrophoresis. A similar decrease of immunoreactivity to each NF subunit is seen in distal nerve segments when noncollagen nerve proteins are immunoblotted. Parallel decreases occur in all three NF proteins, thereby maintaining neurofilament subunit stoichiometry along the neuraxis. The same NF gradient can be detected when the NF contents in nerve branches to the gluteus and gastrocnemius muscles are compared with each other and with those in nerve segments taken from the same proximodistal levels of the parent sciatic nerve. The gradient of NF proteins increases during postnatal development and is readily detected by postnatal day 16. During the same period of development, the heavy NF subunit appears for the first time and is rapidly incorporated throughout the sciatic nerve. Hence, the NF gradient becomes manifest during the development and maturation of the adult form of the axonal cytoskeleton. The basis for the proximodistal gradient of NF proteins in peripheral nerve is presently unknown. The extent of the gradient cannot be accounted for on the basis of diminishing numbers of nerve fibers or increasing amounts of other nerve proteins, e.g., collagen, in distal nerve. An alternative interpretation is that the gradient reflects a low level of NF protein turnover during axonal transport.     

Quantitative comparison between the commercial software STRATOS® by Philips and a homemade software for voxel-dosimetry in radiopeptide therapy

BackgroundTargeted radionuclide therapy is a rapidly growing modality. A few commercial treatment planning systems are entering the market. However, some in-house systems are currently developed for a more flexible and customized dosimetry calculation at voxel-level. For this purpose, we developed a novel software, VoxelMed, and performed a comparison with the software STRATOS.MethodsThe validation of both of them was undertaken using radioactive phantoms with different volume inserts. A cohort of 10 patients was also studied after a therapeutic administration of ¹⁷⁷Lu-labelled radiopeptides. The activity, number of disintegrations, absorbed dose and dose-volume histogram (DVH) were calculated for the phantoms and the kidneys in patients, which were the main critical organs at risk in this study.ResultsIn phantoms the absorbed doses computed with VoxelMed and STRATOS agree within 5%. In patients at the voxel-level the absorbed dose to kidneys (VoxelMed: mean 0.66 Gy/GBq) showed a limited difference of 5%, but with a remarkable range (−40%, +60%) between the two software packages. Voxel-dosimetry allows to estimate the dose non-homogeneities in volumes, which may be evaluated through DVHs.ConclusionThis study demonstrates that a fully 3D voxel-dosimetry with multiple SPECT images is feasible by using home-made or commercial software package and absorbed dose results obtained are similar. The main difference between the studied tools was observed in the activity integration method (effective vs physical half-time to time activity curve tail). We believe that an effective half-time integration method produces a more accurate approximation of clinical uptake and resultant dosimetry.     

Livestock-driven land use change to model species distributions: Egyptian vulture as a case study

Species distribution models (SDMs) are increasingly used to predict species ranges and their shifts under future scenarios of global environmental change (GEC). SDMs are thus incorporating key drivers of GEC (e.g. climate, land use) to improve predictions of species’ habitat suitability (i.e. as an indicator of species occurrence). Yet, most SDMs incorporating land use only consider dominant land cover types, largely ignoring other key aspects of land use such as land management intensity and livestock. We developed SDMs including main land use components (i.e. land cover, livestock and its management intensity) to assess their relative importance in shaping habitat suitability for the Egyptian vulture, an endangered raptor linked to livestock presence. We modelled current and future (2020 and 2050) habitat suitability for this vulture using an organism-centred approach. This allowed us to account for basic species’ habitat needs (i.e. nesting cliff) while gaining insight into our variables of interest (i.e. livestock and land cover). Once nest-site requirements were fulfilled, land use variables (i.e. openland and sheep and goat density) were the main factors determining species’ habitat suitability. Current suitable area could decrease by up to 6.81% by 2050 under scenarios with rapid economic growth but no focus on environmental conservation and rural development. Local solutions to environmental sustainability and rural development could double current habitat suitability by 2050. Land use is expected to play a key role in determining Egyptian vulture's distribution through land cover change but also through changes in livestock management (i.e. species and stocking density). Change in stocking densities (sheep and goats/km²) becomes thus an indicator of habitat suitability for this vulture in our study area. Abandonment of agro-pastoral practises (i.e. below ∼15–20 sheep and goats/km²) will negatively influence the species distribution. Nonetheless, livestock densities above these values will not further increase habitat suitability. Given the widespread impacts of livestock on ecosystems, the role of livestock and its management intensity in SDMs for other (non-livestock-related) species should be further explored.     

Spatial distribution of phytochromes

Phytochromes are chromoproteins which mediate several light responses in plants. Phytochrome proteins are encoded by a gene family which is currently being characterized in several plant species. Analysis of type-specific mutants of two well-characterized members of the family, PhyA and PhyB, indicates that these proteins have distinct functions. Much remains to be learned about the mechanisms by which the phytochromes carry out their distinct and diverse functions. It is hoped that information concerning the localization of phytochromes, at the whole plant and subcellular levels, will aid in elucidating the mechanism of phytochrome function. This review, which summarizes information about phytochrome distribution, has an emphasis on recent reports in which the molecular species of phytochrome are differentiated. However, classical data are also included and reinterpreted using knowledge of the phytochrome family.     

气候变化下中国八种梧桐属树种潜在适生区模拟

中国梧桐属（Firmiana）在世界梧桐属中占比较大，且除梧桐外其余种均为中国特有且分布范围狭窄的植物种，灭绝风险大，研究气候变化对中国梧桐属树种的影响对于维护生物多样性具有重要的意义。结合多时期第六次国际气候耦合模式比较计划（CMIP6）气候变量数据和中国八种梧桐属树种的分布数据，基于R语言kuenm程序包优化的最大熵（Maxent）模型模拟分析中国八种梧桐属树种在多尺度下的潜在适生区，得出梧桐属最适宜的模拟尺度、潜在适生区的面积变化和迁移方向、梧桐属多样性保护关键区域及保护空缺。结果表明：（1）梧桐属最适宜的模拟尺度为亚洲；（2） Maxent模型的接收者操作特征曲线下面积（AUC）值均大于0.9，表明模型对梧桐属潜在适生区预测结果具有较高准确度；（3）气候变化影响下除云南梧桐（Firmiana major）外其它树种的潜在适生区都将在未来有所扩大；（4）中国八种梧桐属树种潜在适生区迁移方向主要为东西向，南北向大跨度迁移较少，纬度变化不大；（5）丹霞梧桐（Firmiana danxiaensis）的稳定潜在适生区最小；（6）中国梧桐属多样性保护关键区域主要分布于广西壮族自治区及云南、广东、海南等省区；（7）中国梧桐属多样性保护空缺区域主要分布于广西壮族自治区中部及海南省北部；（8）梧桐属多样性保护关键区域正在为人造地表所侵蚀。研究分析气候变化对中国八种梧桐属树种的影响及其潜在适生区变化、中国梧桐属多样性保护状态，可为中国梧桐属建立多样性保护廊道提供相关建议，为制定多样性保护规划及相应措施提供参考。     

Computational method for the real-time calculation of the full-body muscle load distribution

A method is described for minimising a quadratic function subject to equality and inequality constraints. This approach is applicable to solving the full-body muscle load distribution problem and calculating joint contact loads. It has been found that this approach can provide the solution on modest computing facilities and in significantly less time than using active set and interior point quadratic programming techniques. Hence the approach is suitable for providing real-time feedback to subjects undergoing biomechanical analysis of muscle, skeletal and joint loadings.